Abstract: Assessment of losses was carried out in various stores of wheat grains in D. I. Khan. A total of 220 samples were collected from different localities in 4 directions (East, West, North and South) of D.I. Khan. The study was conducted by using two methods i.e count and weigh and thousand grain mass methods. During 5 months storage period, average of losses determined by C&W and TGM method were found 3.40 and 6.53%, These losses also include black pointed grains, Broken/Shriveled grains and Green/Immature grains which were computed as 0.61, 3.08 and 0.67% respectively while an average amount of foreign matter was found 30.26%. On an average 1.93% grains were found infested with Tribolium spp., Rhyzopertha dominica and Trogoderma granarium.