Abstract: Background and Objectives: Bacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae (Psa) is one of the most severe bacterial disease of tree species and has been defined as a real pandemic. The Psa aggressiveness has made it very difficult to control with the use of single products, thus an integrated pest management seems to be key to successful control. The aim of this study was to evaluate the inhibitory capacity of 30 plant essential oils (EOs) against Psa. Methodology: The antimicrobial activity of EOs was performed by an in vitro assay based on an evaluation of the bacterial growth in a large volume of nutrient broth supplemented with EOs at different concentrations. The EOs that showed the strongest inhibitive capacities were further tested using a standardized broth microdilution method. Matching the results obtained with both tests, led to the selection of those EOs showing the strongest capacity to inhibit bacterial growth at the lowest concentrations. Results: This study shows that the most effective EOs against Psa were from clove bud (Syzigium aromaticum), thyme (Thymus vulgaris), oregano (Origanum vulgare), cinnamon (Cynnamon zeylanicum) and to a lesser extent, garlic (Allium sativum). Conclusion: This large screening highlight the effectiveness of several EOs to be used for their antibacterial activity against Psa.