Abstract: Background and Objective: Pome fruits are infected by many viruses, it is important to know the way of transmittance and causing agents of disease. To produce virus free propagating material it is necessary to assess the main viruses affecting pome fruits. The research aimed to record the incidence of apple viral infection in commercial fruit tree nursery in Shida Kartli region of Georgia. Materials and Methods: Nine hundred and nine symptomatic and asymptomatic samples were analyzed by means of Double Antibody Sandwich-Enzyme linked Immunosorbent Assay (DAS-ELISA) using commercial kits from BIOREBA AG (Switzerland). Results: According to DAS-ELISA results, 213 from 909 tested samples reacted positively to at least one virus with total incidence of 23.43%. Apple stem grooving virus (10.67%), apple stem pitting virus (8.47%) were found to be frequently presented in infected apple cultivars. Apple chlorotic leaf spot virus has revealed minor spreading -3.3%, but apple mosaic virus infection was not found. Conclusion: Among all tested viruses on apple cultivars, apple stem grooving virus was recorded the highest infection rate and apple chlorotic leaf spot virus was characterized by the lowest distribution.