Abstract: In Indonesia, yellow streak and necrosis symptoms were observed on banana plants. By species-specific PCR, infection with both Banana streak Mysore virus (BSMyV) and/or Banana streak OL virus (BSOLV) was suspected. To confirm whether the DNAs were derived from viral particles or banana endogenous viruses (BEVs), we used combination of two methods: PCR-based assay using long and semi-nested PCR and southern hybridization assay using total DNA. Both of those detection methods revealed that these two samples were infected only by exogenous BSMyV but not BSOLV. This study is the first report of the identification of BSMyV in Indonesia.