Abstract: Five cowpea cultivars, ITO3K -316-1, ITO4K-217-5, IT06K-154-1, IT99K-216-44 and IT03K-369-3, obtained from the International Institute of Tropical Agriculture, Ibadan were screened for resistance to Macrophomina phaseolina infection using two methods of inoculation viz, pouring of spore/mycelia suspension in the soil and wrapping of inoculum meal around wounded lower stem of the seedlings. Cowpea cultivar ITO4K-217-5 was resistant to the pathogen in both inoculation methods while the other four cowpea cultivars i.e., ITO3K-316-1, IT99K-216-44, ITO6K-154-1 and ITO3K-369-3 showed varying degree of susceptibility to the pathogen in both inoculation methods.