Abstract: The aim of the present study was to check the efficacy of carbendazim 50% WP, antagonists and botanicals against Fusarium oxysporum f. sp. psidii associated with rhizosphere soil of guava. Guava (Psidium guajava L.) the apple of tropics is one of the most important fruit in India. The fruit of guava is a good source of vitamin C and pectin. Guava wilt is a serious disease and it recognized as a main causal organism. Isolation and identification of pathogen (Fusarium oxysporum f. sp. psidii) was carried out in the Department of Plant Protection, Allahabad Agricultural institute Deemed University, Allahabad. The radial growth of Fusarium cxysporum f. sp. psidii was fully inhibited at high concentrations like 100, 1000 and 10,000 ppm of cardendazim 50% WP whereas antagonists like Trichoderma spp. Produced maximum inhibition zone (61.91%) followed by Aspergillus niger (61.12%). The radial growth of Fusarium cxysporum f. sp. psidii was also significantly less in neem leaf extract treatment followed by Lantana leaf extract. Management of guava wilt by chemical (systemic fungicide) can be spectacular but this is relatively short-term measure. Eco-friendly management practices, i.e., use of bio-control agents and botanicals was studied in vitro which gave better results and these practices can be economical, long lasting and free from residual side effects.