Abstract: Background and Objective: The regeneration protocol for Jatropha curcas through mature zygotic embryo culture from healthy seeds were studied for large-scale production. This study was specifically undertaken to compare the two kinds modified basal media using zygotic embryos as explants and supplementing both basal media with two growth regulators-an auxin (α-naphthalene acetic acid) and a cytokinin (benzyl adenine) for studying morphogenesis of embryo explants. Materials and Methods: Zygotic embryos from mature seeds of Jatropha curcas were removed and inoculated on modified basal media under aseptic conditions. An auxin (α-naphthalene acetic acid) was used to supplement these media, in addition to a cytokinin (benzyl adenine, BA) either alone or in combination at concentrations between 0 and 10.0 mg L–1. Results: The obtained results revealed that the two basal media used favoured complete in vitro plantlet growth from mature zygotic embryo explants of J. curcas. B5 medium differ notably from MS medium (p = 0.05) in all the growth parameters studied. However, spontaneous plantlet regeneration was observed in the absence of growth regulators after a prolonged period (up to 4 weeks) in culture. Also, in the presence of growth regulators, the simultaneous inclusion of both α-NAA and BA at 0.1 mg L–1 concentrations gave the best response for studying morphogenesis with the embryo explants while the least response was elicited by BA alone at 1.0 mg L–1 concentration on both media. Conclusion: The protocol reported here can be applied for maximum scale propagation of the recalcitrant Jatropha plants for enhanced seed production and the development of a viable conservation programme.