Abstract: In this study the 21 days old seedlings of Pennisetum glaucum were subjected to short term salt stress in order to observe the effect on chlorophyll content, protein and antioxidative enzyme activity (CAT and APX) responses. The aim of the experiment was to evaluate the changes in chlorophyll content and antioxidant enzymes (Catalase and Ascorbate peroxidase). The 21 days old seedlings were subjected to salt stress by supplementing Hoagland’s solution with different concentrations of Sodium chloride (50, 100, 150 and 200 mM). Measurement of chlorophyll content and antioxidant enzyme activity were taken at different time intervals of salt treatment (12, 24, 48, 72, 96 and 120 h). The results showed statistically significant differences in traits for the salt treatment. A significant increase in Catalase activity was observed under all the salt concentrations while no significant activity of Ascorbate peroxidase activity was observed. The protein content and chlorophyll content decreased with increasing salt concentration. This result shows salt stress affects the photosynthesis rate by decreasing chlorophyll content. Catalase enzyme plays an important role in scavenging reactive oxygen species generated due to salt stress in the plant cell.