Abstract: Planttissue culturesare exposed to stresses and stress combinations that they may not have encountered in nature in their long evolution. It is a remarkable reflection on the plasticity of the plant genome that it can decipher and respond to novel in vitro stresses. Today various tissue culture techniques are used to enhance yield of secondary metabolites by trigger stress response like using Elicitors, Precursors and Biotransformation, change in environmental conditions, change in medium constituents etc. The focus of the study is to enhancement of secondary metabolites by triggerthe expression of stress marker genes. This would help in conservation of this species and provide pharmaceutical component in less time and cheap cost.