Abstract: Grape (Vitis venifera), an important fruit crop having ability to give high monitory returns, but area under cultivation is limited due to lack of irrigation resources. Sixteen grape genotypes commercially grown in Western and Northern Maharashtra region were analyzed for anatomical polymorphism to characterize them in relation to Water Use Efficiency (WUE). No of xylem poles in roots, height of palisade mesophyll cells, no of stomata per 100 μm and conducting tissues in petiole were the key anatomical parameters, which will be helpful to select better genotypes for increased water use efficiency and higher yields. Palisade mesophyll height in high yielding stay green genotypes ranged between (103-117 μm) as compared to range of medium yielding stress susceptible genotypes (71-91 μm) indicating the increased photosynthetic rate and higher production due to this important trait. Fewer number and widely spaced stomata’s in resistant plants reduces the chances of evapotranspiration. Genotype Trebbiano Toscano, Chenin Blanc and Syrah were identified as promising varieties for growing in tropical regions with irregular rainfall and water scarcity.