Abstract: Cotton is considered a moderately salt-tolerant crop, however, cotton varieties differ in the level of salt- tolerance and high salinity reduces growth, yield and fiber quality in a different extent. The objectives of this study were to determine the response of selected CRS accessions to salt stress and to identify salt-tolerant CRS accession(s) or individual plants within these accessions that potentially could be used as parental material for further investigation of salt tolerance in cotton. Seedling of four putative salt-tolerant (M-9044-0031, M-9044-0061, M-9044-0140 and M-9044-0150) and three salt-sensitive CRS accessions (M-9044-0060, M-8744-0091 and M-8744-0175) plus Acala 1517-88, Deltapine 50 and TAM 94-L25 were grown under hydroponic culture with two different salt concentrations (150 and 200 mM NaCl) and control. The study was carried out under completely randomized experimental design. Significant differences were observed among cotton genotypes for Shoot (SL) and Root Length (RL) and Shoot Dry Weights (SDW) and Root Dry Weights (RDW) reduction with the increasing salt levels. It was concluded that CRS lines, M-9044-0060, M-9044-0061 and M-8744-0091, may provide additional insights and parental material for breeding for salt tolerance in upland cotton. In addition, selected single plants from salt-tolerant CRS lines were transferred to pots to obtain seeds for further investigation of salt tolerance in cotton.