Abstract: Investigations were carried out on the non-target effects of two new fungicides, Amistar and Score (Syngenta, Mumbai, India) in terms of phytotoxicity. These fungicides are found to be generally non phytotoxic at or below the recommended dose for field application (2.2 μg (a.i.) mL-1). At higher concentrations, the extent of phytotoxicity of Azoxystrobin and Difenoconazole varied with host genotype. Among the test plants, Vigna catjung was most sensitive to both the fungicides for all the studied parameters like seed germination, shoot elongation, root elongation, number of lateral roots initiated and may serve as good indicator of phytotoxicity of these fungicides. Both the fungicides at their different concentrations significantly decreased community respiration and gross primary productivity. However, the net primary productivity was significantly increased by Azoxystrobin treatment up to 0.0073 μg (a.i.) mL-1 and Difenoconazole up to 0.0014 μg (a.i.) mL-1 concentrations. Treatment of leaf tissue with Azoxystrobin resulted in electrolyte leakage as measured by increased electrical conductivity (EC). The increase in EC was pronounced with the increase in fungicide concentration and incubation period. The negative EC values obtained in the Difenoconazole treatment may be due to fast and efficient uptake of the fungicide from the ambient solution by the leaf tissue.