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American Journal of Plant Physiology

Year: 2006 | Volume: 1 | Issue: 1 | Page No.: 41-53
DOI: 10.3923/ajpp.2006.41.53
Differential Response of Scavenging of Reactive Oxygen Species in Green Gram Genotype Grown under Salinity Stress
Neelam Misra, Mohd. Saquib Ansari and Ajay Kumar Gupta

Abstract: Salinity stress affects the metabolism of plant cells leading to severe crop damage and loss of yield. Oxidative stress is one of the major causes of salinity that may be responsible for the tissue damage. The present endeavor is based on the immediate responses on enzymatic and non-enzymatic to salinity-induced oxidative stress in two high yielding green gram (Phaseolus aureus) cvs. salt tolerant (T-44) and salt sensitive (SML-32). Under salinity stress, the salt tolerant cv. T-44 showed the higher activity of ROS scavenging enzyme, catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), Glutathione Reductase (GR), glutathione-S-transferase (GST) and enhanced levels of ascorbate (ASC) and glutathione (GSH), than the sensitive cv. SML-32. While, superoxide dismutase (SOD), monodehydroascrobate reductase (MDAR) and dehydroascorbate reductase (DHAR) were lower in cv. T-44 than cv. SML-32, it indicated lesser extent of membrane damage (lipid peroxidation) and lower levels of H2O2. The high levels of CAT activity indicate efficient scavenging of H2O2, which is produced more by non-enzymatic means than via SOD in cv. T-44. These findings suggest that planned action of enzymatic as well as non-enzymatic ROS scavenging machineries are essential to overcome the salinity-induced oxidative stress in green gram.

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How to cite this article
Neelam Misra, Mohd. Saquib Ansari and Ajay Kumar Gupta, 2006. Differential Response of Scavenging of Reactive Oxygen Species in Green Gram Genotype Grown under Salinity Stress. American Journal of Plant Physiology, 1: 41-53.

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