Abstract: Softening of tissue is a serious problem of mango. It is thought that it may be due to deficiency of minerals specially calcium. Low uptake, movement and distribution of calcium which is controlled by the movement of water through transpiration stream could be one of the main reasons for the incidence of softening in mango. Therefore, the relationship of leaf and fruit transpiration rates with the incidence of softening of tissue in mango cultivars. Dashehari (susceptible) and Chausa and Langra (free from softening tissue) were investigated. Leaf transpiration rates were similar in both the cultivars, however the fruit transpiration rates were significantly higher in cultivar Chausa followed by Langra. Artificially induced variations in fruit transpiration rates using perforated polythene covers, paper bags and vaseline coating of fruits also resulted in significant changes in the occurrence of softening tissue in cv. Dashehari along with alternation in chlorophyll, TSS (°Brix) and carotenoid content in different treatments. The significant and negative relationship (r = -0.436 and -0.521) observed between the fruit transpiration rates and softening tissue suggests that the lower fruit transpiration rates in cultivar Dashehari are a varietal specific trait, which results in slower movement of water and mineral to the fruits from the soil/leaf leading to the development of softening of tissue in mango.