Abstract: Survey on the nematode of the superfamily Aphelenchoidea revealed the presence of a new species of Seinura associated with rooten pine wood from Bishnupur, Manipur, North East India. The nematodes were extracted from collected samples by Baermann funnel technique. Seinura manipurensis sp. nov. is characterized by a body length of 534.57-645.29 μm, spear = 17.3 μm, spear without stylet knobs, a = 26.64-30.9, b = 3.74-4.82, c = 4.66-5.94, c’ = 8.66-9.5, V = 68.60-70.50, post uterine sac 2.75-4.37 times vulval body widths long and arrangement of oocytes in a single row.