Abstract: Labor pain and its relieving methods is one of the anxieties of mothers having a great impact on the quality of care during delivery as well as the patients' satisfaction. The propensity of using non-medicinal pain relief methods is increasing. The present study aimed to compare the effect of Acupressure at two GB-21 and SP06 points on the severity of labor pain. In this quasi-experimental single blind study started on December 2010 and ended on June 2011 in which 150 primiparous women were divided into three groups of Acupressure at GB-21 point, Acupressure at SP-6 point and control group. The intervention was carried out for 20 min at 3-4 and 20 min at 7-8 cm dilatation of Cervix. The pain severity was measured by Visual Analog Scale before and immediately, 30 and 60 min after the intervention. Then, the data were statistically analyzed. No significant difference was found among the 3 groups regarding the pain severity before the intervention. However, the pain severity it was reduced at 3-4 and 7-8 cm dilatation immediately, 30 and 60 min after the intervention in the two intervention groups compared to the control group (p<0.001). Nonetheless, no statistically significant difference was observed between the two intervention groups (p = 0.93). The results of the study showed that application of Acupressure at two GB-21 and SP-6 points was effective in the reduction of the severity of labor pain. Therefore, further studies are recommended to be performed on the application of Acupressure together with non-medicinal methods.