Abstract: Background and Objective: In fact, a big variety of fruits which differ in shape, color, taste and nutritional value are available in the market and many are widely used for production of fermented beverages. In this study, the physicochemical characteristics (pH, °Brix and acidity), total phenol content and antioxidant activity of alcoholic-fermented noni (Morinda citrifolia L.) must were evaluated. Methodology: The noni wine was analyzed using physicochemical analysis (volumetric titrations, potentiometric and refractometry methods). The total polyphenol content and antioxidant activity by visible molecular absorption spectroscopy and the chemometric analysis by supervised pattern recognition techniques. Results: The effect of alcoholic fermentation on the pH and °Brix in presence of Saccharomyces cerevisiae (experimental group) produces a decrease in these parameters and an increase of the acidity; these changes are significant at p<0.05. The total polyphenol content varied during the alcoholic fermentation. The highest content was produced in the 4th week containing 612 mg GAE L–1. The antioxidant activity (DPPH) as measured by the inhibition coefficient (IC50) reflects an increase in this activity and in the 4th week increased upto 18.75%. In the first 4 weeks, there was a better correlation between the total polyphenol content and the IC50 radical scavenging capacities. A satisfactory SLDA result for the alcoholic fermentation according to pH, °Brix and acidity, total phenols content and antioxidant activity was obtained, in which the correct classification was 100%. Conclusion: Alcoholic fermentation induces significant changes in composition, mainly the total phenol content.