Abstract: This study was conducted to access the extraction yield of antioxidant and their activity from Egyptian tomato waste. Conventional and novel assisted extraction methods (ultrasound and microwave) using different hydrophilic and lipophilic solvents at different times and temperatures for optimizing extraction conditions were used. Total phenolics, flavonolids and lycopene were individually determined in different solvent extracts. Activity of their antioxidant compounds was performed using radical scavenging activity and β-carotene-linoleic acid bleaching methods. It was found that novel assisted extraction methods gave higher yield of antioxidants and antioxidant activity. In addition preliminary High Performance Liquid Chromatography (HPLC) was used to identify the antioxidant components. Increase in temperature and extraction time had a significant effect on antioxidant yields and their activity. Eco-friendly solvents such as water and ethyl lactate gave better results than the others. This study demonstrates the possibility of recovering high amounts of natural antioxidant compounds with higher antioxidant activity from tomato wastes using eco-friendly solvents and novel assisted extraction methods.