Abstract: Production of hydrolysates by Actinomucur elegans during soy-protein deep-liquid fermentation was a promising prospects for future applications in the protein hydrolysate industry. And the optimal incubation conditions for protease production by Actinomucor elegan, which have been investigated in several studies. The hydrolyzing conditions were optimized by the single factor and orthogonal tests. Five single factor of important effect on the DH and PCL were explored. The optimum condition was determined as fermentation time 5 days, substrate concentration 3%, initial pH 6.0, liquid medium volume 80 (mL bottle-1), Inoculums concentration 4.5 (v/v). The maximum DH can reach 38.29%.