Abstract: The suitability of sugarcane juice for the in vitro culture of bananas and plantains was investigated in this study. Two concentrations of sugar cane juice 5, 10% and 30 g L-1 laboratory sucrose were used. There was also a control medium without carbon source. Explants were prepared from field grown sword suckers. The preparation of the explants involved, sterilization with 70% ethanol, followed by 1% Sodium hypochlorite+1% tween 20. The explants were cultured at 26°C, under 16 h photoperiod, light intensity of 3000 lux and a relative humidity of 60%. Plants that were cultured on 5% sugarcane juice had higher average fresh weight values of 16.6 g per plant compared to those that were cultured on the 30 g L-1 sucrose and 10% sugarcane juice which had fresh weight of 15.6 g per plant and 11.0 g per plant, respectively. Oniba had higher fresh weight on medium supplemented with 5% sugarcane juice compared to the two other cultivars. In terms of dry weight, ‘Oniaba’ again had the highest value of 7.2 g per plant which was obtained on medium supplemented with 5% sugarcane juice. Plants that were cultured on 5% sugarcane juice were better in terms of shoot height and number of shoots per plant than those cultured on 30 g L-1 sucrose and the differences between the 5% sugarcane juice and the other treatments were statistically different. The various carbon sources and their concentrations also affected the water potential of the media. Medium supplemented with 10% sugarcane juice had the lowest water potential of -0.8 MPa compared to the control medium that had a water potential of -0.1 MPa. Medium supplemented with 5% sugarcane juice and 30 g L-1 sucrose had in a medium water potential of -0.3 MPa. Five percent sugarcane juice was found to be a better substitute for laboratory grade sucrose for the in vitro propagation of Musa sp.