Abstract: The purpose of this study was to characterization of xylanase producing Fusarium solani isolate and optimization of cultural conditions for xylanase enzyme production. Screening of Fusarium solani isolate was based on the diameter of the clear zone formation in oat spelt xylan agar plates, Fusarium solani isolate F7 was selected and optimized for xylanase enzyme production using cheaper substrate like wheat straw, rice straw, rice bran and wood husk. Maximum enzyme activity was observed in wheat straw (78.32 U mL-1). Optimum pH and temperature for xylanase activity were found to be 5.5 and 30° at 3% substrate concentration. In purification step, 75% ammonium sulphate saturation was found to be suitable giving maximum xylanase activity. Purified xylanase yielded single band with a molecular weight of 89 kDa. The use of wheat straw as a major carbon source is particularly valuable because oat spelt xylan is very expensive, The Fusarium solani F7 isolate proved to be a promising microorganism for xylanase production.