Abstract: The present study was conducted to monitor the presence of antibiotic residues in poultry meat. Swab Test on Animal Food (STAF) employing Bacillus subtilis as test organism on nutrient agar with 0.4% dextrose at pH 7, was used. A spore suspension of 2x107 spores mL-1 was used in 100 mL of nutrient agar to make STAF plates. A total of 100 tissue samples (33 liver, 33 kidney and 34 muscles) were collected from local market of Rawalpindi and Islamabad. Thirteen (39.4%) liver, 9 (27.3%) kidney and 7 (20.6%) muscle samples were detected positive for antibiotic residues. Antibiotic sensitivity and Minimum Inhibitory Concentration (MIC) of Oxytetracycline, Gentamicin, Neomycin, Enrofloxacin, Chloramphenicol, Amoxicillin, Benzyl Penicillin, Streptomycin and Tylosin against Bacillus subtilis was determined to be 0.6100, 0.0549, 0.1525, 0.1525, 0.0023, 0.0143, 0.0095, 0.0380 and 0.0023 μg, respectively. It is concluded from the study that STAF test can be used for the preliminary detection of antimicrobial residues in poultry tissues.