Abstract: Zoonoses are common infections when there is close contact between humans and animals. In Nigeria, where safety at work is poorly regulated and enforced, most workers do whatever they consider appropriate safe work place practices. Thus, 263 abattoir workers across five North Central (NC) states in Nigeria were evaluated on their knowledge of leptospirosis, work place attitudinal practices and other risks that could favor spread of Leptospira among them. Findings showed that most workers 252 (95.8%) were ignorant of leptospirosis. Other identified risk factors for infection included age of workers, occupation, illiteracy and risks within, such as inadequate protective gear and outside the workplace, such as rodents in homes. All these factors gave a prevalence of 226 (89.7%) Leptospira antibodies in the abattoir workers which they probably contracted through un-mindful handling of infected animal fluids and tissues. Surveys of Knowledge, Attitudes, Risk Factors and Practices (KARP) is a common strategy for collecting information to assess safe work environments among high risk populations and is a good starting point for prevention and control programs for individuals at risk of infection by zoonotic agents, especially Nigerian abattoir workers.