Abstract: Serological screening of dogs for brucellosis is not a routine practice in veterinary clinics in Jos. Hence, this study was conducted to determine the prevalence of brucellosis in dogs in Jos North and South Local Government Areas (LGA) of Plateau State. A total of 350 sera samples were collected from dogs presented to the clinics. The samples were analysed using Rose Bengal Plate Test (RBPT) which gave a prevalence of 113 (32.3%). The RBPT positive samples were then subjected to Competitive Enzyme-linked Immunosorbent Assay (c-ELISA) and 33 (29.2%) of them were positive. Sero-prevalence increased with age using the c-ELISA as 1 (20.0%) of the 5 dogs between 0-6 months, 2 (22.2%) of the 9 dogs between 6-11 months and 30 (30.3%) of the 99 dogs above 11 months were positive. Based on sex, 6 (28.6%) of the 21 male dogs and 27 (29.3%) of the 92 female dogs were positive for c-ELISA. Breed-based prevalence recorded 27 (31.0%) of 87 exotic breeds, 1 (16.7%) of 6 cross-breeds a nd 5 (25.0%) of 20 indigenous breeds of dogs assayed. Dogs in Jos South had a higher prevalence of 29 (29.6%) of the 98 dogs tested than those in Jos North which had 4 (26.7%) of the 15 dogs tested. However, there was no statistical significance between brucella infection and age, sex, breed or location of dogs sampled (p>0.05). This study indicates that canine brucellosis is prevalent in Jos thus, enlightenment and awareness programmes to educate the dog owners should be carried out by the relevant authorities.