Abstract: There is continuing controversy over the association between serum level of 25-hydroxyvitamin D (S-25 OHD) and cardiovascular risk factors. The aim of the present study was to examine the association between serum lipid profile and S-25 OHD levels in Bangladeshi young female garment factory workers with exclusive indoor lifestyle. A total of 200 subjects (aged 18-36 years) were randomly selected. Fasting S-25 OHD, Total Cholesterol (T-C), Triacylglycerol (TAG), High-density Lipoprotein Cholesterol (HDL-C), Low-density Lipoprotein Cholesterol (LDL-C), Very-low-density Lipoprotein Cholesterol (VLDL-C), LDL-C/HDL-C ratio were measured. About 92% of the subjects were below 30 years of age. A high prevalence (88.5%) of hypovitaminosis D (S-25 OHD <50 mmol L-1) was observed and there was no significant association between S-25 OHD and T-C. The acceptable level of T-C was highly prevalent (92%) in the subjects. The prevalence of high T-C and LDL-C were 1.5 and 2.5%, respectively. The HDL-C level (<1.04 mmol L-1) was predominantly low (about 90% ) in the subjects. Data indicated that subjects with S-25 OHD level both <50 and >50 mmol L-1 had no influence on T-C, LDL-C, LDL/HDL ratio, VLDL-C and TAG levels. Anthropometric variables such as Body Mass Index (BMI), bicep and tricep skinfolds, Mid-upper Arm Circumference (MUAC), hip and waist circumference had a significant (p<0.005) association with T-C and TAG. The results of the study do not support any association of S-25 OHD levels with unfavourable lipid profile. Further intervention studies in different subject groups are warranted to evaluate whether increasing vitamin D intake with supplementation or increased exposure to sunlight will improve the metabolic cardiovascular risk factors profile.