Abstract: A herd of cattle, flock of sheep and goats and Prison inmates in Sokoto Prison farm were investigated for evidence of brucellosis. Serum samples obtained from cattle, sheep, goats and humans were serologically assessed using Rose Bengal Plate Test (RBPT) Serum tube Agglutination Test (SAT) and competitive Elisa (compelisa). Similarly, Milk Ring Test (MRT) was carried out using Milk sample. Twenty-eight Prison inmates were tested for Brucella antibodies. An overall prevalence of 32.20% was recorded in the herds. 16 out (40%) of the 40 cows were positive while 2(20%) of the 10 bulls tested were positive and 1(2%) out of the 3 females calves was founds to be positive. Sokoto Gudali was the breed with the highest prevalence (37.58%), while white Fulani recorded a prevalence of 25%. None of the 3 Azuwarq breed was positive to Milk Ring Test. An overall prevalence of 22.35% was recorded in sheep while an overall prevalence of 30.76% was recorded in goats. Two (7.14%) out of the 28 Prison inmates were found to be positive. It is recommended that milk and milk product from seropositive animals should not be consumed. Extra hygiene measures are also recommended among others.