Abstract: The multidrug resistant Klebsiella is becoming an increasingly common clinical entity and has presented a global clinical problem. The present research aimed to observe the prevalence of infection and multidrug resistance pattern of Klebsiella in Chittagong region, Bangladesh. A total of 99 Klebsiella isolates associated with Klebsiella infection were isolated and identified according to the standard microbiological method from 502 individual patients. The most prevalent infection caused by Klebsiella isolates was urinary tract infection and male were more vulnerable to Klebsiella infection than female. Female of age 31-41 years group were found to be very much prone to Klebsiella associated infection whereas male has highest percentage on age group greater than 50 which was 58%. The sensitivity tests to Klebsiella were carried out with 5 antibiotics such as, Azythromycin, Amoxicillin, Cefexime, Nalidixic acid and Ciprofloxacin on Mueller-hinton agar by modified Kirby-bauer disc diffusion technique. Among the Klebsiella isolates, 92, 87, 60 and 42% were susceptible to Ciprofloxacin, Azythromycin, Cefixime and Nalidixic acid, respectively. Nearly 81% Klebsiella isolates exhibited resistance to amoxicillin and 58% isolate to Nalidixic acid. Azithromycin and Ciprofloxacin showed tremendous level of sensitivity to Klebsiella spp. The present study revealed the incidence of resistance to available antibiotics is currently relatively high, which implies a total lack of antibiotics for treatment of life-threatening infections caused by this MDR Gram-negative ‘superbug’.