Abstract: Moringa oleifera (Moringaceae) is a bush of African savannah, commonly known as Drum stick used in folk Medicine for the treatment of rheumatic pain, analgesic activity, antipyretic activity. The aims of present study were Evaluation of Membrane Stabilizing Potential and Antimicrobial Activity stem bark of Moringa oleifera (Moringaceae) against Selected Microbes. In Diffusion assay (Plate method) of extracts of M. oleifera depends on the diffusion of an antibiotic from a vertical cylinder or cavity through the solidified agar layer of petri plate to an extent such that growth of added microorganism is presented entirely in circular area or “zone” around the cavity. In this method zone of inhibition is determined. HRBC membrane stabilization method used for evaluation of in vitro anti-inflammatory activity. The results of HRBC Stabilization in vitro anti-inflammatory activity of Moringa oleifera of extracts showed good inhibitory activity in petroleum ether extract as % inhibition of Haemolysis 5, 12, 20 and 33% in 10, 25, 50 and 100 μg mL-1 concentration, respectively. Chloroform extract and methanol extracts of Moringa oleifera showed moderate to good zone of inhibition against selected microorganisms compared with standard as Gentamicin. Analgesic and anti-inflammatory effects of flavonoids, steroids and tannins have been reported hence the anti-inflammatory effect produced by these extracts may be predictable due to the flavonoids and steroids. The HRBC membrane stabilizing property of Moringa oleifera was found to be promising and also exhibited good antimicrobial activity.