Abstract: In this survey which was taken in 7 horse riding clubs in Ahvaz, the rate of Salmonella infection in apparently healthy horses were investigated. The Fecal samples were collected from 129 horses and cultured between April 2011 to January 2012. The sampling and culture were done 5 times in two week intervals for each horse. Isolation and identification of Salmonella from fecal samples were done according to routine bacteriological and PCR methods. The results of this survey showed that 3 (2.32%) horse was infected with Salmonella and it was identified as Salmonella enteritidis. Among the separated Salmonellas 93% were resist to Ampicillin, 16.5% to Tetracycline and 40.5% to Kanamycin, but all of them were sensitive to Gentamicin in this antibiotic resistance test.