Abstract: New pyrrole derivatives (2,3) were synthesized by condensation method and synthesized compounds were characterized by IR, 1H NMR, 13C NMR, mass and elemental analysis. The novel pyrrole derivatives were screened for anticoagulant activity against Activated Partial Thromboplastin Time (APTT) and Prothrombin Time (PT) coagulation assays and compound (3) is highly response for anticoagulant activity compared with compounds (1) and (2) and standard Heparin at concentration (60 μg mL-1) against APTT assay. The compound 3 showed highly significant anticoagulant effect and could be considered as leads for further investigations.