Abstract: The present study deals with the effect of Gynandropsis pentaphylla (leaves and seeds) extracts (acetone, benzene, methanol, ether and water) on the production of extra and intracellular protein in 6 bacteria and 4 fungi. Effects of different type of extracts were done through the estimation of intra and extra cellular proteins content. Acetone extract of seeds inhibited the extra cellular protein synthesis at maximum level (0.072±0.02 mg L-1) in Bacillus subtilis NCIM 2010, followed by benzene extract of seeds which inhibited the synthesis of extra cellular protein (0.083±0.03 mg mL-1) in Escherichia coli NCIM 2064. Water extract of seeds suppressed the extra cellular protein synthesis at maximum level (0.296±0.08 mg mL-1) in Aspergillus niger NCIM 501. Intracellular protein synthesis was highly (0.285±0.12 mg mL-1) affected after the addition of methanol extracts of leaves in Staphylococcus aureus NCIM 2120. Intracellular protein synthesis was highly (0.169±0.09 mg mL-1) affected after the addition of benzene extracts of seed in Penicillium notatum NCIM 747. Sucrose, D-mannose and L-rhamnose were observed in the water extracts of leaves and seeds, when screened using TLC. Water extracts of leaves showed arginine, proline and methionine, while seeds showed proline and tyrosine.