Abstract: Plant biotechnology is an attractive means for improving cotton, its use requires an efficient regeneration system from somatic tissues of cotton plants. Cotton is an important economic and fibre crop, grown in 70 countries in the world. Somatic embryogenesis and plant regeneration are fundamental to the genetic improvement of cotton using biotechnology and genetic transformation. Browning and subsequent death of the cultured explants are major problems for many tissue culture systems. In this study, different explants and various concentrations of PGRs were tested in order to minimize excretion of phenolic compound and their inhibitory effects in cotton tissue culture system. Cotyledons and meristematic shoot tips had the minimal excretion of phenolic compound, also in medium with 27.12 μM 2,4-D and 8.87 μM BA phenolic compound was the least. Meristematic shoot tips in medium with 0.492 μM IBA and 0.929 μM KIN regenerated whole plantlets without any excretion phenolic compound. Therefor, in this study inhibitory effects due to excretion of phenolic compound in tissue culture media were minimum.