Abstract: Dermatophyte infections constitute an important public health problem in different parts of the world. Dermatophytosis, commonly referred to as ringworm, is a superficial fungal infection caused by filamentous fungal species belonging to three anamorphic genera, Trichophyton, Microsporum and Epidermophyton. Dermatophytes choose areas of safety for their growth, such as superficial keratinous tissues, to escape from host defense mechanisms. Although, dermatophytes can be isolated worldwide, many species are only encountered in geographically restricted areas. This study was carried out to identify the etiological agents of tinea capitis in north Gaza strip Palestine and its mode of transmission. This study was carried out for three years from September 2009 to August 2012. A total of 100 samples of suspected tinea capitis were collected heads (scalp), primarily diagnosed by the dermatologists in three primary health care centers in North Gaza strip. Cultural characteristics, morphological characteristics and physiological tests (e.g., urease test, NaCl 3% test, hair perforation test, rice grain test) were used for the identification of the causative dermatophytes. The present results revealed that the most common dermatophytes caused tinea capitis in North Gaza strip were Microsporum canis (92.5%) and Trichophyton mentagrophytes (7.5%). The domestic animals played an important role in transporting the disease which resulted in prevalence of tinea capitis in North Gaza strip compared to the neighboring countries.