Abstract: Background and Objective: Indonesia is one of the chief producers of palm oil of the world market, but all production is collected in one place. This research will include later whether there is a correlation between seedlings that are more adaptable of water stress in nurseries with field planting. This study was aimed to know the response of the growth of palm oil seedlings and ABA content to Vedagro fertilizer treatment and water stress. Materials and Methods: The experiment was conducted in the field experiment station of the Agricultural Faculty of Darma Agung University Medan, Indonesia, with ±28 meters above sea level. The research is done in May, 2019 until August, 2019. This research uses the Completely Randomized Block Design Factorial Method consisting of 2 treatment factors, that is Vedagro consists of 3 levels, V1 = 5.0 g baby polybag–1, V2 = 10.0 g and V3 = 15.0 g baby polybag–1 each, while water stress with 4 levels namely W0 = 0.2 L baby polybag–1 day–1, W1 = 0.2 L baby polybag–1 days–2; W2 = 0.2 L baby polybag–1 days–3; W3 = 0.2 L baby polybag–1 days–4. Results: The results showed that the higher the dose of Vedagro, the higher the growth of seedlings, on the contrary, the lack of water cause the growth of seedling decreased but the ABA content increased. Conclusion: Vedagro up to 15 g baby polybag–1 increases significantly only to plant height. Water stress decreases significantly in plant growth but ABA content increase significantly 0.0022 ppm cm–2 leaf area.