Abstract: Aim: Production of new barley (Hordeum vulgare L.) genotypes with stable grain yield is an important challenge in variable and harsh climatic conditions such semi-arid zones. Methodology: For this purpose, twenty-nine 6-row barley genotypes, 26 barley anther culture-derived doubled haploid lines obtained from F2 plants of 2 biparental crosses, along with 3 parental cultivars were tested for grain yield and stability level at five semi-arid environments in Algerian highlands barley grown areas. Several stability parameters such, regression coefficient (bi), deviation from regression (S2di), Pinthus’ coefficient (R2), environmental variance (S2i), coefficient of variation (CV%), Wricke’s genotypic ecovalence (Wi), Shukla’s stability variance (σi2), heterogeneity variance (%HV), incomplete correlation (%IC) and Plaisted’s stability Parameter (P), were used to assess the stability of each genotype. Results: Twenty eight genotypes showed a wide adaptability (bi = 1) and only a single line (DH40) showed a specific adaptation (bi<1). About 10 genotypes showed yield stability over the environments studied (S2di = 0, low values of Wi, %HV, %CV, %IC, σi2 and high R2). Upon these 10 genotypes, eight gave high yields (Express, DH11, DH14, DH15, DH21, DH30, DH39 and DH10). The DH26, DH65 and DH2 lines have given the best yields but are unstable. The majority of doubled haploid lines derived from Tichedrett×Express hybrid, showed more stability than the local parent and a yield equal to the general mean yield. Conclusion: Significant correlations were obtained within the two groups of parameters of static stability concept and dynamic stability concept, indicating the possibility of using a single stability parameter per group.