Abstract: This study was carried out to investigate the effect of NaCl on callus necrosis, the percentage of survival calli and shoot regeneration in rice cultivars, namely Khao Dawk Mali 105, Chai Nat 1, Suphan Buri 1 and Pathum Thani 1, after 60, 90, 120 and 150 days of exposure. After 150 days of exposure, calli showed a decrease in the survival percentage and relative fresh weight against the increasing NaCl concentration. The survival percentages of 55, 52, 44 and 56 were respectively observed in Khao Dawk Mali 105, Chai Nat 1, Suphan Buri 1 and Pathum Thani 1. The relative fresh weights were found to be 0.10, 0.75, 0.23 and 0.01, respectively. Following 120 day exposure, 175 mM NaCl was found to inhibit growth of all rice cultivars. Calli exposed to NaCl exhibited lower percentage of shoot regeneration than control. The percentage of shoot regeneration in Khao Dawk Mali 105, Chai Nat 1, Suphan Buri 1 and Pathum Thani 1 calli receiving 150 mM NaCl were 36, 32, 36 and 28%, respectively. Rooting of selected-shoots was also observed and the findings revealed that, after 30 days of culture, shoots can produce normal roots.