Abstract: Field experiments were conducted to determine the effects of the inhibitor of gibberellin (GA) biosynthesis on the growth and yield of rice (Oryza sativa L.) grown by no tillage cultivation with single basal fertilization of Controlled-Availability Fertilizer (CAF) to the nursery box. Inabenfide (4'-chloro-2'-(α-hydroxybenzyl)-isonicotinanilide: IBF) was used as an inhibitor of GA biosynthesis. There was no difference in the plant age between the control and IBF treatment. However, the length of IBF-treated plants is significantly longer than the control plants during the growth period. The number of tillers was similar between both treatments until the maximum tiller number stage, after that, it significantly increased in IBF-treated plants. The IBF treatments increased the number of panicles by 24.5% compared with the control but decreased the number of grains per panicle. The IBF treatment did not affect the total number of grains, the reduction in grain number per panicle was compensated by the increased number of panicles. The percentage of ripened grains and the one-thousand-grain weight in IBF-treated plants were similar to those of the control. When all yield components were taken into consideration, no difference in grain yield was found between the IBF treatment and control groups. The present report indicates that IBF works well for promoting tillering in no-tillage rice cultivation with a single basal fertilization of CAF to the nursery box.