Abstract: The presented study was conducted to study the effects of BAP and its combinations with 2, 4-D and sucrose concentrations on androgenesis of broccoli cv. ‘Arcadia’. The MS Basel media supplemented with AC (0.5 g L-1) and 3% sucrose was used. The experiment was laid out in Completely Randomized Design (CRD) using 3 replicates. The effects of BAP in combination with 2, 4-D and sucrose concentrations on androgenesis of broccoli (cv Arcadia) anther cultures were investigated. The MS basal media supplemented with activated charcoal (AC) (0.5 g L-1) was used. The medium complemented with BAP (1 mg L-1) plus 2, 4-D (0.5 mg L-1) significantly increased the percentage of anthers developing embryos (90%). Increasing 2, 4-D concentration (1 mg L-1) significantly decreased the percentage of anthers formed embryos (10%). Regarding the regeneration of the formed embryos, the culture medium containing BAP (1 mg L-1)+2, 4-D (0.5 mg L-1) or BAP (1 mg L-1)+2, 4-D (1 mg L-1) produced the high percentages of embryos induced callus. The maximum percentage of embryos developed into plantlets were obtained with culture medium with BAP (1 mg L-1)+2, 4-D (0.5 mg L-1) followed by BAP (1 mg L-1) and 2, 4-D (1 mg L-1). Concerning sucrose concentration, the culture medium with low sucrose concentration (20 g L-1) enhanced the percentage of anthers developed embryos. The higher sucrose concentrations (50 and 60 g L-1) increased percentages of dead anthers and anthers formed callus and decreased the percentage of embryos developing plantlets. BAP in combinations with 2, 4-D and low concentrations of sucrose can be successfully used to perform embryos and/or callus from the callus induction and embryos development either to callus or plants.