Abstract: Four diverse genotypes of Stevia rebaudiana Bertoni were included for Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis. Total proteins were analyzed through slab type SDS-PAGE. Based on SDS-PAGE, specific bands were suggested to be used for identifying Stevia rebaudiana Berton. This method has the advantages of simplicity, high sensitivity and good accuracy and the SDS-PAGE proved to be a powerful tool for differentiating Stevia rebaudiana Bertoni varieties.