Abstract: Background and Objective: Free radicals in the body increased along with aging. Free radical oxidation in fat tissue will produce malondialdehyde (MDA). Amount of antioxidant vitamins was required to reduce the oxidation rate. The objective of study was to determine the effect of date palm seed steeping to free radical products (MDA) and antioxidant vitamins (vitamin E) in alloxan induced rat model. Materials and Method: A quasi-experimental research with pre- and post-test design was used. Thirty rats were randomly distributed into 6 groups. From 1st-4th groups were given with 0.25, 0.5, 0.75 and 1 g kg–1 BW of date seed steeping. The 5th group was a positive control (vitamin C 1 g kg–1 BW) and the 6th group was a negative control. Before the date palm seed steeping was given, rats induced by alloxan. Blood was taken before and after treatment for MDA and vitamin E measurement. The data were analyzed by one-way ANOVA and continued by LSD post hoc test. Results: The study showed that date palm seed steeping can decrease MDA levels and increase vitamin E levels in all treatment groups significantly (p<0.05), but did not occur in negative control group (p>0.05). Conclusion: The results showed that date palms seed could reduce the levels of MDA and increase the vitamin E levels.