Abstract: Probiotics are live microorganisms beneficially affect the host by improving the properties of the indigenous microbial balance when ingested in certain numbers. Synbiotics are the combination of probiotics and prebiotics. They are able to alter the composition of human gut flora towards a predomination of probiotics benefitting human physical and mental health. Defecation situation is able to authentically reflect gut health. The aim of the present study was to investigate the effect of daily ingestion of synbiotics for 20 days on the defecation of female and male volunteers in different ages. Results revealed that, after 20 days-supplementation of synbiotics, 61.67% volunteers’ defecation discipline was improved. 56.67% volunteers found gut significantly relaxed. Volunteers’ stool smell, color, frequency and diarrhea symptoms were modified to various extents. Synbiotics tended to have greater impact on the defecation of elderly and female volunteers.