Abstract: Inflammation is a physiological response to an injury and disruption by the external factor. VipAlbumin® is a one of the supplement that has anti-inflammatory activity. This study wants to know the effect of VipAlbumin® to macrophage, pro-inflammatory cytokines and transcriptional factor NF-κB. The experiment was done by cultured cells from healthy mice spleen in RPMI with 10% FBS, stimulant anti-CD3 and LPS, 2-Mercaptoethanol and VipAlbumin®’s concentration were 0, 0.33, 33.3 and 3333.3 μg mL-1. This study showed that VipAlbumin® did not only decrease the number of macrophage cells, TNF-α and IFN-γ cytokines produced by CD4+ T cells and IL-6 cytokines produced by macrophage cells, but also suppress NF-κB activation in CD4+ and CD8+T cells and macrophage cells significantly (p<0.05) compared to control. Those results proved that this supplement can be used to cure many kinds of disease which is caused by inflammation.