Abstract: Inducing ovary to mature using hormones known to be a great tool to enhance the production of larvae. This study shows the effect of reproductive steroid hormones, 17α-hydroxyprogesterone and 17α-hydroxypregnenolone with different doses on the ovarian maturation stages of orange mud crab, Scylla olivacea. This study period was 60 days. Total of 90 immature female orange mud crab S. olivacea were used in this study where 18 immature female S. olivacea acted as a control without the injection, while the other four treatments T1D1, T1D2, T2D1 and T2D2 each having 18 immature female S. olivacea and injected laterally in fifth abdominal segment with different dose of 17α-hydroxyprogesterone and 17α-hydroxypregnenolone with each hormone have two treatments with the dosage of 0.01 and 0.1 μg g-1 b.wt. at a day 0, 10, 20, 30, 40, 50 and 60th. Every ten days, 3 individual were sacrificed from the control and the treatment. The findings in this study indicate that 17α-hydroxyprogesterone and 17α-hydroxypregnenolone injection should be evaluating more dosage before being use as a practical way of improving ovarian development in commercial operations.