Abstract: Background and Objective: Maluku water is a higher in marine biodiversity and having species richness of laor worms (Polychaetes). These worms used as food source by local people and they believe it’s good for health. Understanding and assessment of biochemical compositions are important, thus, the purpose of this study was to determine the fatty acid and amino acid of laor worms in Tobelo coastal water and compared with others studies. Materials and Methods: Samples were taken using fishing net and then placed in plastic boxes. Some part of samples were fixed with 5% of formaldehyde solution then immediately observed the morphological under stereo microscope for taken photographs. Other samples were short cut then dried at 55°C in oven until constant weight. The dried samples were analyzed for total mineral, amino acid and fatty acid contents. Results: The result revealed that laor worms were varied in morphology. Laor worms with light green and dark green body color were dominant appeared followed by yellow-red and red-brown. Calcium and magnesium were found in samples as well as several metals. Total concentrations of Saturated Fatty Acid (SFA), monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) were 15.42, 12.22 and 37.87%, respectively. About 15 amino acids were determined included 8 essential amino acids (41.31%) and 7 non-essential amino acids (53.34%). Conclusion: Overall there was a variation in composition of fatty acids and amino acids in laor worms from place to place. Totally, fatty acid and amino acid concentrations of laor worms in this study were higher than other coastal water in Indonesia.