Abstract: Background and Objective: Legumes of the genus Medicago have particular ecological and agro-pastoral importance in the Mediterranean regions. In order to develop local species by identifying and selecting cultivars adapted, populations of Medicago truncatula and Medicago laciniata were evaluated at the experimental farm of INRAA (Baraki) in 2015/2016 cropping season. Materials and Methods: Ten populations of M. truncatula and M. laciniata from different regions of Djelfa were used. The study is based on pheno-morphological and agronomic traits. ANOVA and principal components analysis (PCA) were performed on the complete set of the data. Results: ANOVA showed significant differences between populations for the majority of studied parameters. Also, the two principal components (PC1 and PC2) explained 72.73% of the total variation for morphological variation and 79.43% for agronomic evaluation trial. Conclusion: Large variability among natural populations of M. truncatula and M. laciniata was observed for all studied traits. This variability can be exploited in fodder breeding programs to select an adapted plant material for arid and semi-arid area.