Abstract: Background and Objective: Acacia nilotica is a species of nilotica in the Fabaceae family. Phytoconstituents in the pods extract may be responsible for the antimicrobial activity of the plant. The purpose of this study was to formulate and evaluate hydrophilic ointment of Pods extract microbiologically. Materials and Methods: Medicated Ointment was formulated as 5% dry methanolic extract of 50 g of powdered material of Acacia nilotica pod, hydrophilic bases were used. Commercially available Fucidin ointment was used as a standard for this study. Six formulae were prepared and evaluated for various pharmaceutical parameters such as rheological properties, stability, pH and external characters as well as anti-microbial property against different pathogenic micro-organism. Inhibition zone (in mm) of different ointments was also measured. Two-way ANOVA was used to analyze the obtained data. Results: Each of ointment mixture of extract and citric acid and ointment of extract alone were better than each of ointments of citric acid, standard fucidin ointment and non-medicated one. Conclusion: Acacia nilotica pods have satisfactory results pharmaceutically as well as biologically and promote good antimicrobials properties.