Abstract: Background and Objective: The concentration of biochemical metabolites in follicular fluid of the ovary fluctuates considerably with cycle stage, follicle size and follicle status and the presence of corpus luteum. Therefore, this study was designed to study dynamics of follicular fluid composition in relation to follicular size and corpus luteum in dromedary camels. Methodology: Two hundred ovaries were collected at slaughterhouse from hundred female dromedary camels. The ovaries were classified into ovaries with CL (CL-bearing group) and ovaries without CL (non-CL bearing ovaries). Follicular fluid of small, medium and large sized follicles were aspirated and analyzed for hormonal (estradiol-17β, progesterone, insulin and leptin) and antioxidant (nitric oxide (NO), ascorbic acid and glutathione (GSH), beside biochemical concentrations (glucose and cholesterol). Results: The large sized follicles contained significantly (p<0.05) higher leptin concentrations than medium and small ones in ovaries with or without CL. Furthermore, the concentration of Insulin was significantly higher (p<0.05) in FF obtained from large and small follicles in CL-bearing ovaries than those found in non-CL bearing ovaries. Furthermore, insulin concentrations were significantly higher (p<0.05) in the FF obtained from large and small follicles in CL-bearing ovaries than those obtained in non-CL bearing ovaries. However, GSH levels were significantly higher (p<0.05) in the FF obtained from large follicle compared to other follicles in CL-bearing ovaries. Conclusion: The FF composition was modified depending on the ovarian status. Presence or absence of the corpus luteum played a crucial role in follicular fluid composition of dromedary camels. Further studies are required to investigate the different pathways affecting camel follicular physiology.