Abstract: Background and Objective: Propanil (PRO) is an acylanilide herbicide used to control barnyard grass and broad leaf weeds. This study evaluated the impact of PRO in streptozotocin (STZ)-induced diabetic rats. Materials and Methods: Diabetes was induced in rats by a single injection of STZ (60 mg kg–1 intraperitoneal, i.p.). The PRO was administered at the dose of 200 mg kg–1 (i.p. for 7 days), to both normal and diabetic rats. Results: The plasma levels of triglyceride, aspartate aminotransferase, creatinine and renal malondialdehyde level (an index of lipid peroxidation) were significantly increased in diabetic rats. In addition, the levels of high density lipoprotein-cholesterol, superoxide dismutase were depressed in the STZ-treated rats compared to the control group. The co-exposure of PRO increased absolute organ weight, catalase, glutathione peroxidase and malondialdehyde levels. Conclusion: Taken together, exposure to PRO exacerbated oxidative stress in renal tissue of diabetic rats.