Abstract: Background and Objective: Cotton is the backbone of Indian textile industry, accounting for 75% of total fibre consumption in textile sector and 38% of the country’s export. To increase the yield of cotton and in order to meet the demand of cotton industry, development of appropriate cropping system and nutrient management strategies to cotton has becomes crucial. Therefore, study on the effect of intercrops, inorganic fertilizers, biofertilizers and tree leaf extract spray on seed cotton yield was taken up. Materials and Methods: Intercropping system viz., C1-Cotton sole, C2-Cotton+Blackgram, C3-Cotton+ Greengram and C4-Cotton+Cluster bean were allotted to main plot. The intercrops residues were incorporated at 65 DAS. The subplot consisted of six treatments viz., N1-100% RDF (Recommended Dose of fertilizer-80:40:40 kg NPK/ha), N2-75 % RDF, N3-75% RDF+biofertilizers, N4-75 % RDF+5% Morinda leaf extract spray, N5-75% RDF+5% Vilvam leaf extract spray, N6-75% RDF+5% Annona leaf extract spray. The experiments were laid out in a split plot design with three replications. Leaf extracts were sprayed at 60 and 80 DAS. The biofertilizers include Azospirillum+Phosphobacteria+Silicate solubilizing bacteria each at 2.6 kg ha–1. Results: Intercropping system, nutrient management and botanicals treatment showed significant response interms of seed cotton yield. Among the different intercropping system and nutrient management, cotton + blackgram intercropping with integrated nutrient management involving application of 75% RDF+seed treatment and soil application of biofertilizers recorded higher seed cotton yield (1619 and 1715 kg ha–1 during winter 2007 and Summer 2007-08, respectively). Conclusion: Cotton+blackgram intercropping system with application of 75% RDF+seed treatment and soil application biofertilizers may be recommended for higher seed cotton yield.