Abstract: Objective: The study investigated the potentials of methanol extract of Nauclea latifolia root bark in modulating blood glucose concentration, lipid profile and haematological parameters in alloxan induced diabetic rats. Materials and Methods: The acute toxicity study was carried out with 18 mice while the study on lipid profile and haematological parameters were carried out with 30 male Wister Albino rats using standard reagents and assay protocols. The rats were randomly distributed into six groups of five rats each with group 1 as the normal control that was free from diabetes and received no treatment. Group 2 and 3 were diabetic rats treated with 1.5 mL normal saline and 2.5 mg kg–1 b.wt., of glibenclamide, respectively while group 4-6 were diabetic rats treated with 100, 200 and 400 mg kg–1 b.wt., of the extract, respectively for 14 days. Results: The result of the acute toxicity study revealed that the methanol extract was relatively safe within the maximum dose tested as no adverse reactions nor death was recorded. The extract exhibited hypoglycaemic activity in the diabetic rats comparable with the standard drug (glibenclamide) as the diabetic rats treated with graded doses of the extract showed significant (p<0.05) decrease in the blood glucose concentrations relative to the diabetic untreated rats with low dose having the highest hypoglycaemic activity. The diabetic rats had significant (p<0.05) decrease in packed cell volume count and haemoglobin concentrations, however, administration of the extract significantly (p<0.05) increased packed cell volume count and haemoglobin concentrations, respectively. Significant (p<0.05) increase in WBC count and no significant increase in red blood cell count (p>0.05) observed in the diabetic control relative to the normal control were significantly reversed when treated with the extract. The extract showed significant antihyperlipidaemic activity, reducing total cholesterol and low density lipoprotein concentrations significantly with no significant increase in high density lipoprotein and decrease in triacylglycerol concentration, respectively. Conclusion: The findings of this study have validated the use of the extract in the management of diabetes as it positively modulated blood glucose concentration, lipid profile and haematological parameters.